Recombinant Human NKG2C&CD94 Protein,His-Avi Tag

Description :
Synonyms NKG2C&CD94;; CD94; Source Human NKG2C&CD94 Protein is expressed from HEK293 with His tag and Avi tag at the N-Terminus. It contains Glu98-Leu231(NKG2C)&Ser34-Ile179(CD94).[Accession | P26717(NKG2C)&Q13241-1(CD94)] Molecular Weight The protein has a predicted MW of 15.3kDa (NKG2C)&17.9kDa (CD94). Due to glycosylation, the protein migrates to 30-50 kDa based on Tris-Bis PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by SDS-PAGE and HPLC. Activity SPR Data: Human NKG2C&CD94, His Tag immobilized on CM5 Chip can bind Human HLA-E*01:03 Complex Tetramer, His Tag with an affinity constant of 1.65 M as determined in SPR assay . Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.