Recombinant Biotinylated Cynomolgus BAFF/TNFSF13B/CD257 Trimer Protein,His-Avi-Flag Tag

Description :
Synonyms CD257; BLyS;BAFF; TNFSF20 Source Biotinylated Cynomolgus BAFF/TNFSF13B/CD257 Trimer Protein is expressed from HEK293 with His tag and Flag tag and Avi tag at the N-Terminus. It contains Thr141-Leu285.[Accession | A0A2K5V2X4] Molecular Weight The protein has a predicted MW of 54.2 kDa. Due to glycosylation, the protein migrates to 55-60 kDa based on SDS-PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by SDS-PAGE Activity ELISA Data: Immobilized Biotinylated Cynomolgus BAFF (Trimer) , His Tag at 5g/ml (100l/well) on the streptavidin precoated plate (5g/ml). Dose response curve for Human BAFFR, hFc Tag with the EC50 of 17.4ng/ml determined by ELISA. Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.