Recombinant Human GPA33/A33 Protein,His-Avi Tag

Description :
Synonyms A33; GPA33;Glycoprotein A33; MGC129987 Source Human GPA33/A33 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Ile22-Val235.[Accession | Q99795-1] Molecular Weight The protein has a predicted MW of 26.5 kDa. Due to glycosylation, the protein migrates to 28-40 kDa based on SDS-PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by SDS-PAGE and HPLC. Activity ELISA Data: Immobilized Human GPA33, His Tag at 1g/ml (100l/well) on the plate. Dose response curve for Anti-GPA33 Antibody, hFc Tag with the EC50 of 4.8ng/ml determined by ELISA. Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.