Recombinant Human Integrin alpha V beta 3 (ITGAV&ITGB3) Heterodimer Protein,hFc Tag

Description :
Synonyms beta3; Integrin alpha V beta 3;V3; ITGAV&ITGB3 Source Human Integrin alpha V beta 3 (ITGAV&ITGB3) Heterodimer Protein is expressed from HEK293 with hFc tag at the C-Terminus. It contains Phe31-Val992(ITGAV)acidic tail & Gly27-Asp718(ITGB3)basic tail.[Accession | P06756-1(ITGAV)&P05106-1(ITGB3)] Molecular Weight The protein has a predicted MW of 131.90 kDa (ITGAV)&101.79 kDa (ITGB3). Due to glycosylation, the protein migrates to 140-160 kDa (ITGAV)&105-130 kDa (ITGB3) based on SDS-PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by SDS-PAGE and HPLC. Activity ELISA Data: Immobilized Human ITGAV&ITGB3, hFc Tag at 1g/ml (100l/well) on the plate. Dose response curve for Biotinylated Anti-alpha V beta3 Antibody, hFc Tag with the EC50 of 16.9ng/ml determined by ELISA. Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.