Recombinant Biotinylated Human NKp30/NCR3/CD337 Protein,His-Avi Tag

Description :
Synonyms NCR3; LY117;CD337; 1C7 Source Biotinylated Human NKp30/NCR3/CD337 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Leu19-Thr138.[Accession | AAH52582] Molecular Weight The protein has a predicted MW of 20 kDa. Due to glycosylation, the protein migrates to 26-45 kDa based on SDS-PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by SDS-PAGE and HPLC. Activity ELISA Data: Immobilized Biotinylated Human NKp30, His Tag at 1g/ml (100l/well) on the streptavidin precoated plate (5g/ml). Dose response curve for Anti-NKp30 Antibody, hFc Tag with the EC50 of 6.2ng/ml determined by ELISA . Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.