Recombinant Human DcR1/TRAILR3 Protein,His Tag

Description :
Synonyms CD263; LIT; DCR1-TNFR; TRAILR3 Source Human DcR1/TRAILR3 Protein is expressed from HEK293 with His tag at the C-Terminus. It contains Tyr24-Ala236.[Accession | Q05D71] Molecular Weight The protein has a predicted MW of 23.5 kDa. Due to glycosylation, the protein migrates to 60-80 kDa based on Tris-Bis PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by SDS-PAGE and HPLC. Activity SPR Data: Human DcR1, His Tag immobilized on CM5 Chip can bind Human TRAIL, No Tag with an affinity constant of 0.57 nM as determined in SPR assay . Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.