Recombinant Human TRAIL R1/DR4/TNFRSF10A Protein,His-Avi Tag

Description :
Synonyms DR4; TRAIL-R; TNFRSF10A; TRAIL R1 Source Human TRAIL R1/DR4/TNFRSF10A Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Pro34-Asn239.[Accession | O00220] Molecular Weight The protein has a predicted MW of 24.7 kDa. Due to glycosylation, the protein migrates to 25-30 kDa based on Tris-Bis PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by SDS-PAGE and HPLC. Activity SPR Data: Human TRAIL R1, His Tag captured on CM5 Chip via anti-his antibody can bind Human TRAIL, No Tagwith an affinity constant of 1.02 nM as determined in SPR assay . Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.