Recombinant Biotinylated Human VEGF R2/KDR Protein (Primary Amine Labeling) ,mFc Tag

Description :
Synonyms CD309; KDR; VEGFR; VEGFR2; VEGFR-21; FLK1; KRD1; Ly73 Source Biotinylated Human VEGF R2/KDR Protein (Primary Amine Labeling) is expressed from HEK293 with mFc (IgG1) tag at the C-Terminus. It contains Ala20-Glu764.[Accession | P35968-1] Molecular Weight The protein has a predicted MW of 110 kDa. Due to glycosylation, the protein migrates to 135-160 kDa based on SDS-PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by SDS-PAGE and HPLC. Activity ELISA Data: Immobilized Human VEGF165, No Tag at 2g/ml (100l/Well) on the plate. Dose response curve for Biotinylated Human VEGF R2, mFc Tag with the EC50 of 0.14g/ml determined by ELISA. Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.