Tabolism, signal transduction, amino acids, tetrapyrrole binding, carboxypeptidase activity, amylase activity
Tabolism, signal transduction, amino acids, tetrapyrrole binding, carboxypeptidase activity, amylase activity, and cell cycle regulation have been upregulated. Carboxypeptidase can hydrolyze polypeptides into amino acids. Chlorophyll belongs to the category of tetrapyrrole derivatives. enrichment evaluation of KEGG metabolic pathways (Fig. two: g ) revealed that following BR spraying, the expression of protein processing-related genes in the endoplasmic reticulum was substantially upregulated. Protein processing in the endoplasmic reticulum consists of glycosylation, hydroxylation, acylation, and disulfide bond formation, of which one of the most significant is glycosylation. Nearly all proteins synthesized within the endoplasmic reticulum are finally glycosylated. Genes connected to starch and sucrose metabolism had been drastically LIMK2 site upregulated in CAC (BR spraying for 24 h). Genes connected to ubiquitin-mediated proteolysis had been significantly upregulated in CAD (BRsJin et al. BMC Genomics(2022) 23:Page 7 ofFig. 2 a The number of differential genes up- or downregulated by the 4 comparison combinations (CAA vs. CAK, CAB vs. CAK, CAC vs. CAK, and CAD vs. CAK). b Venn diagram of four comparative combinations. c Column chart of GO enrichment analysis of upregulated differentially expressed genes in c CAA vs. CAK, d CAB vs. CAK, e CAC vs. CAK, and f CAD vs. CAK. g , g CAA vs. CAK upregulation in the bubble map of differentially expressed genes by KEGG enrichment evaluation. KEGG enrichment evaluation bubble chart of upregulated genes in h CAB vs. CAK, i CAC vs. CAK, and j KEG CAD vs. CAKsprayed for 48 h). Ubiquitin-mediated proteolysis produces amino acids. GO and KEGG enrichment analyses showed that after spraying BRs onto tea leaves, genes associated to sugar, starch, chlorophyll metabolism, the cell cycle, signal transduction, and amino acid synthesis have been upregulated.qRT-PCR evaluation of DEGsTo confirm the gene expression patterns detected around the transcriptome dataset, qRT-PCR evaluation was performed to figure out the mRNA expression of BAK1, BES1, BSU1, SPS, SBE, protochlorophyllide oxidoreductase (POR), DFR, CycD3, threonine synthase (TS), glutamine synthetase (GS), arginine decarboxylase (ACD), and inducer of C-repeat-binding element expression (ICE) inside the 5 samples (Fig. three). The expression profiles in the single genes detected in qRT-PCR analysis coincided with these detected inside the RNA-seq datasets.Exogenous spraying of BR onto tea leaves promotes the upregulated expression of genes involved within the BR signal transduction pathwayKEGG enrichment 5-HT Receptor Agonist supplier annotation revealed that 26 genes are involved inside the BR signal transduction pathway (Fig. four: 1). KEGG analysis showed that compared with CAK (BR spraying for 0 h), the expression levels of BRI1, BAK1, transmembrane kinase four (TMK4), 14-3-3, abscisic acid G-protein coupled receptor (GPCR), BSU1, BES1, and BES1-interacting myc-like two (BIM2) which might be connected to BR signal transduction have been upregulated immediately after BR spraying (for 3 h, 9 h, 24 h, and 48 h), but the highest gene expression levels varied amongst time points, which may be as a result of the various sequences of signal transduction.Exogenous spraying of BR promotes cell division, theanine synthesis, and improved expression of genes connected to cold resistance in tea leavesKEGG enrichment and annotation revealed that a lot of cyclin genes in tea leaves had been upregulated by BR spraying (Fig. 4: 2). Additionally, 3 genes for theanine synthesis and a single gene associated to cold resistance wer.
