Recombinant Human VLDLR Protein,His Tag

Description :
Source Human VLDLR Protein is expressed from HEK293 with His tag at the C-terminal. It contains Gly28-Ser797.[Accession |P98155-1] Molecular Weight The protein has a predicted MW of 85.9 kDa. Due to glycosylation, the protein migrates to 115-125 kDa based on Tris-Bis PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by Tris-Bis PAGEandHPLC Activity SPR Data: Human VLDLR, His Tag immobilized on CM5 Chip can bind Human PCSK9, His Tag with an affinity constant of 0.72 nM as determined in SPR assay Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.