Recombinant Human VEGFR1/FLT-1 Protein,His-Avi Tag

Description :
Source Recombinant Human VEGF R1/FLT-1 Protein is expressed from HEK293 with His tag and Avi tag at the C-terminal. It contains Ser27-Asn756.[Accession |P17948-1] Molecular Weight The protein has a predicted MW of 85.1 kDa. Due to glycosylation, the protein migrates to 100-120 kDa based on Tris-Bis PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by Tris-Bis PAGE Activity ELISA Data: Immobilized Human VEGF R1, His Tag at 2g/ml (100l/well) on the plate. Dose response curve for Biotinylated Human VEGF121, His Tag with the EC50 of 36.5ng/ml determined by ELISA ELISAData:Immobilized Human PGF, hFc Tag at 0.5g/ml (100l/Well) on the plate. Dose response curve for Human VEGF R1, His Tag with the EC50 of 12.8ng/ml determined by ELISA. Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.