Recombinant Human IL-13Ra1 Protein,hFc Tag

Description :
Source Human IL-13Ra1 Protein is expressed from HEK293 with hFc tag at the C-terminal. It contains Ala27-Thr343.[Accession |P78552] Molecular Weight The protein has a predicted MW of 63.4 kDa. Due to glycosylation, the protein migrates to 78-90 kDa based on Tris-Bis PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by Tris-Bis PAGEandHPLC Activity ELISA Data:Immobilized Human IL-13, His Tag at 5g/ml (100l/Well). Dose response curve for Human IL-13Ra1, hFc Tag with the EC50 of 4.7g/ml determined by ELISA SPR Data:Human IL-13Ra1, hFc Tag captured on Protein A chip, can bind Human IL-13, His Tag with an affinity constant of 53.1nM as determined in a SPR assay. Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.