Recombinant Human HLA-A*02:01&B2M&NY-ESO-1 (SLLMWITQC) Tetramer Protein,His-Avi Tag

Description :
Source Human HLA-A*02:01&B2M&NY-ESO-1 (SLLMWITQC) Tetramer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus,tetramer is assembled by biotinylated monomer and streptavidin. It contains Gly25-Thr305(HLA-A*02:01),Ile21-Met119(B2M) and SLLMWITQC peptide. [Accession |A0A140T913(HLA-A*02:01)&P61769(B2M)&SLLMWITQC] Molecular Weight The protein has a predicted MW of 258 kDa. Due to glycosylation, the protein migrates to 260-265 kDa under Non reducing (N) condition based on Tris-Bis PAGE result. 内毒素Endotoxin 1EU /g by the LAL method. Purity > 95% as determined by Tris-Bis PAGEandHPLC Activity ELISA Data:Immobilized Human HLA-A*02:01&B2M&NY-ESO-1 (SLLMWITQC) Tetramer, His Tag at 5g/ml (100l/Well) on the plate. Dose response curve for Anti-HLA-A*02:01&B2M &NY-ESO-1(SLLMWITQC) Antibody, hFc Tag with the EC50 of 2.3ng/ml determined by ELISA. SPR Data: Anti-HLA-A*02:01&B2M&NY-ESO-1 (SLLMWITQC) Antibody, hFc Tag captured on CM5 Chip via Protein A can bind Human HLA-A*02:01&B2M&NY-ESO-1 (SLLMWITQC) Tetramer, His Tag with an affinity constant of 0.09 nM as determined in SPR assay Formulation Supplied as 0.22m filtered solution in 20mM PB, 500mM NaCl (pH 7.4).