Recombinant Biotinylated Human IGF1R/CD221 Protein,His-Avi Tag

Description :
Source Biotinylated Human IGF1R/CD221 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus. It contains Glu31-Asn932.[Accession |P08069] Molecular Weight The protein has a predicted MW of 105.8 kDa (alpha subunit) and 23 kDa (beta subunit). Due to glycosylation, the protein migrates to 110-120 kDa (alpha subunit) and 52-55 kDa (beta subunit) based on Tris-Bis PAGE result. 内毒素Endotoxin 1EU per g by the LAL method. Purity > 95% as determined by Tris-Bis PAGEandHPLC Activity ELISA Data:Immobilized Anti-IGF1R Antibody at 0.5g/ml (100l/well) on the plate. Dose response curve for Biotinylated Human IGF1R, His Tag with the EC50 of 48.0ng/ml determined by ELISA. ELISA Data:Immobilized Biotinylated Human IGF1R, His Tag at 5g/ml (100l/well) on the streptavidin precoated plate (5g/ml). Dose response curve for Human IGF-I, hFc Tag with the EC50 of 0.73g/ml determined by ELISA. Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.