Recombinant Cynomolgus CD73/NT5E Protein,His Tag

Description :
Source Cynomolgus CD73/NT5E Protein is expressed from HEK293 with His tag at the C-terminal. It contains Trp27-Cys554.[Accession |EHH53214.1] Molecular Weight The protein has a predicted MW of 59.6 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on SDS-PAGE result. 内毒素Endotoxin 1EU per g by the LAL method. Purity > 95% as determined by SDS- PAGEand HPLC Activity ELISA Data: Immobilized Cynomolgus CD73, His Tag at 1g/ml (100l/Well) on the plate. Dose response curve for Anti-CD73 Antibody, hFc Tag with the EC50 of 10.8ng/ml determined by ELISA.. Bioactivity:Measuredbyitsabilitytohydrolyzethe5′-phosphategroupfromthesubstrate adenosine-5′-monophosphate (AMP). The specific activity is > 10000 pmol/min/g. Formulation Lyophilized from 0.22m filtered solution in 20mM Tris, 120mM NaCl (pH 7.5). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.