Recombinant Human CD40 Ligand/TNFSF5 Trimer Protein,His-Flag Tag

Description :
Source Human CD40 Ligand/TNFSF5 Trimer Protein is expressed from HEK293 with His tag and Flag tag at the N-terminal. It contains Met113-Leu261.[Accession |P29965]. Molecular Weight The protein has a predicted MW of 52.4 kDa, Due to glycosylation, the protein migrates to 55 kDa based on SDS-PAGE result. 内毒素Endotoxin 1EU per g by the LAL method. Purity > 95% as determined by SDS-PAGEandHPLC Activity ELISA Data: Immobilized Human CD40 Ligand Trimer, His Tag at 1g/ml (100l/Well) on the plate. Dose response curve for Human CD40, hFc Tag with the EC50 of 1.29g/ml determined by ELISA. Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.