Recombinant Human CD47 Protein,hFc Tag

Description :
Source Human CD47 Protein is expressed from HEK293 with hFc tag at the C-terminal. It contains Gln19-Pro139.[Accession |Q08722-1] Molecular Weight The protein has a predicted MW of 40.5 kDa. Due to glycosylation, the protein migrates to 55-65 kDa based on SDS-PAGE result. 内毒素Endotoxin 1EU per g by the LAL method. Purity > 95% as determined by SDS-PAGEandHPLC Activity ELISA Data: Immobilized Human SIRP alpha V8, His Tag at 1g/ml (100l/Well) on the plate. Dose response curve for Human CD47, hFc Tag with the EC50 of 21.2ng/ml determined by ELISA. SPRData: Human CD47, hFc Tag captured on Protein A chip via Anti-His Antibody can bind Human Sirpa V2, His Tag with an affinity constant of 13.8nM as determined in SPR assay Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.