Recombinant Human CD3E/CD3 epsilon monomer Protein,hFc Tag

Description :
Source Human CD3E/CD3 epsilon monomer Protein is expressed from HEK293 with hFc tag at the C-terminal. It contains Asp23-Asp126(C119S, C122S).[Accession |P07766] Molecular Weight The protein has a predicted MW of 36.1 kDa. Due to glycosylation, the protein migrates to 35-55 kDa based on SDS-PAGE result. 内毒素Endotoxin 1EU per g by the LAL method. Purity > 95% as determined by SDS-PAGE Activity ELISA Data: Immobilized Human CD3E, hFc Tag at 1g/ml (100l/Well) on the plate. Dose response curve for Biotinylated Anti-CD3 Antibody, hFc Tag with the EC50 of 33.5ng/ml determined by ELISA. Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.