Recombinant Human CD83 Protein,hFc Tag

Description :
Source Human CD83 Protein is expressed from HEK293 with hFc tag at the C-terminal. It contains Thr20-Ala143.[Accession |Q01151-1] Molecular Weight The protein has a predicted MW of 40.8 kDa. Due to glycosylation, the protein migrates to 55-65 kDa based on SDS-PAGE result. 内毒素Endotoxin 1EU per g by the LAL method. Purity > 95% as determined by SDS-PAGEandHPLC Activity ELISA Data: Immobilized Human CD83, hFc Tag at 0.5g/ml (100l/Well) on the plate. Dose response curve for Biotinylated Anti-CD83 Antibody, hFc Tag with the EC50 15.3ng/ml determined by ELISA. Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.