Recombinant Biotinylated Human CD3E&CD3D/CD3 epsilon&CD3 delta Protein (Primary Amine Labeling) ,His Tag

Description :
Source Biotinylated Human CD3E&CD3D/CD3 epsilon&CD3 delta Protein (Primary Amine Labeling) is expressed from HEK293 with His tag at the C-terminal. It contains Asp23-Glu120(CD3E) & Phe22-Ala105(CD3D).[Accession |P07766(CD3E)&P04234(CD3D)] Molecular Weight The protein has a predicted MW of 17.9 kDa (CD3E) and 16.7 kDa (CD3D). Due to glycosylation, the protein migrates to 25-40 kDa based on SDS-PAGE result. 内毒素Endotoxin 1EU per g by the LAL method. Purity > 95% as determined by SDS-PAGEand HPLC Activity ELISA Data: Immobilized Anti-CD3 Antibody, mFc Tag at 5g/ml (100l/well) on the plate. Dose response curve for Biotinylated Human CD3E&CD3D, His Tag with the EC50 of 0.72g/ml determined by ELISA. Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.