Recombinant Biotinylated Mouse FcRn Protein,His-Avi Tag

Description :
Synonyms FcRn alpha chain; FCGRT; FcRn; FCGRT&B2M Source Biotinylated Mouse FcRn Protein is expressed from HEK293 His tag and Avi tag at the C-Terminus. It contains Ser22-Ser297 (FCGRT) and Ile21-Met119 (B2M).[Accession | P01887&Q61559] Molecular Weight The protein has a predicted MW of 47.1 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on SDS-PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by SDS-PAGE and HPLC. Activity SPR Data:Biotinylated Mouse FcRn, His Tag captured on CM5 Chip via anti-his antibody can bind Trastuzumab with an affinity constant of 1.22 nM as determined in SPR assay . Formulation Lyophilized from 0.22 m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.