Recombinant Mouse CD27/NFRSF7 Protein,mFc Tag

Description :
Source Mouse CD27/NFRSF7 Protein is expressed from HEK293 with mFc (IgG1) tag at the C-terminal. It contains Pro24-Arg182.[Accession |P41272-1] Molecular Weight The protein has a predicted MW of 44.3 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Tris-Bis PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by Tris-Bis PAGEand HPLC Activity ELISA Data: Immobilized Mouse CD27, mFc Tag at 1g/ml (100l/Well) on the plate. Dose response curve for Mouse CD27 Ligand, hFc Tag with the EC50 of 35.5ng/ml determined by ELISA. Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.