Recombinant Biotinylated Human CD155/PVR Protein,His-Avi Tag

Description :
Source Recombinant Biotinylated Human CD155/PVR Protein is expressed from HEK293 with His tag and Avi tag at the C-terminal. It contains Trp21-Asn343.[Accession |P15151-1] Molecular Weight The protein has a predicted MW of 38 kDa. Due to glycosylation, the protein migrates to 55-70 kDa based on Tris-Bis PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by Tris-Bis PAGEandHPLC Activity ELISA Data: Immobilized Biotinylated Human CD155, His Tag at 1g/ml (100l/well) on the streptavidin precoated plate (5g/ml). Dose response curve for Human TIGIT, hFc Tag with the EC50 of 8.8ng/ml determined by ELISA ELISAData: Immobilized Biotinylated Human CD155, His Tag at 5g/ml (100l/well) on the plate. Dose response curve for Human DNAM-1, hFc Tag with the EC50 of 4.8g/ml determined by ELISA. Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.