Recombinant Mouse CD47 Protein,hFc Tag

Description :
Source Mouse CD47 Protein is expressed from HEK293 with hFc tag at the C-terminal. It contains Gln19-Lys140.[Accession |Q61735-1] Molecular Weight The protein has a predicted MW of 40.60 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Tris-Bis PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by Tris-Bis PAGEandHPLC Activity SPR Data: Mouse CD47, hFc Tag captured on CM5 Chip via Protein A can bind Mouse SIRP alpha, His Tag with an affinity constant of 28.33 nM as determined in SPR assay Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.