Recombinant Cynomolgus/Rhesus macaque CD47 Protein,His Tag

Description :
Source Cynomolgus/Rhesus macaque CD47 Protein is expressed from HEK293 with His tag at the C-terminal. It contains Gln19-Glu141.[Accession |F7A802] Molecular Weight The protein has a predicted MW of 15 kDa. Due to glycosylation, the protein migrates to 45-65 kDa based on Tris-Bis PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by Tris-Bis PAGEandHPLC Activity ELISA Data: Immobilized Cynomolgus/Rhesus macaque CD47, His Tag at 5g/ml (100l/well) on the plate. Dose response curve for Human SIRP alpha, hFc Tag with the EC50 of 0.52ug/ml determined by ELISA SPR Data:Cynomolgus SIRP alpha, His Tag immobilized on CM5 Chip can bind Cynomolgus /Rhesus macaque CD47, His Tag with an affinity constant of 63.27 nM as determined in SPR assay Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.