Recombinant Biotinylated Human CD3E/CD3 epsilon 1-27 peptide Protein,hFc-Avi Tag

Description :
Source Biotinylated Human CD3E/CD3 epsilon 1-27 peptide Protein is expressed from HEK293 with hFc tag and Avi Tag at the C-terminal. It contains Asp23-Thr48.[Accession |P07766] Molecular Weight The protein has a predicted MW of 31.3 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Tris-Bis PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by Tris-Bis PAGEand HPLC Activity ELISA Data: Immobilized Anti-CD3 Antibody, mFc Tag at 1g/ml (100l/well) on the plate. Dose response curve for Biotinylated Human CD3E 1-27 peptide, hFc Tag with the EC50 of 5.9ng/ml determined by ELISA. Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.