Recombinant Biotinylated Human IL-2R alpha/CD25 Protein,His-Avi Tag

Description :
Source Biotinylated Human IL-2 R alpha/CD25 Protein is expressed from HEK293 with His tag and Avi tag at the C-terminal. It contains Glu22-Cys213.[Accession |P01589] Molecular Weight The protein has a predicted MW of 24.6 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Tris-Bis PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by Tris-Bis PAGE > 95% as determined by HPLC Activity ELISA Data: Immobilized Anti-CD25 Antibody, hFc Tag at 0.2g/ml (100l/well) on the plate. Dose response curve for Biotinylated Human IL-2 R alpha, His Tag with the EC50 of 11.9ng/ml determined by ELISA. Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.