Recombinant Human CD73/NT5E Protein,His Tag

Description :
Source Recombinant Human CD73/NT5E Protein is expressed from HEK293 with His tag at the C-terminal. It contains Trp27-Lys547.[Accession |P21589-1] Molecular Weight The protein has a predicted MW of 58.8 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Tris-Bis PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by Tris-Bis PAGEandHPLC Activity ELISA Data:Immobilized Human CD73, His Tag at 0.5g/ml (100l/well) on the plate. Dose response curve for Anti-CD73 Antibody, hFc Tag with the EC50 of 40.6ng/ml determined by ELISA. EnzymeActivity: Measuredbyitsabilitytohydrolyzethe5′-phosphategroupfromthe Substrateadenosine-5′-monophosphate (AMP). The specific activity is >15000 pmol/min/g Formulation Lyophilized from 0.22m filtered solution in 20mM Tris, 120mM NaCl (pH 7.5). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.