Recombinant Human CD96/TACTILE Protein,His Tag

Description :
Source Recombinant Human CD96/TACTILE Protein is expressed from HEK293 with His tag at the C-terminal. It contains Val22-Met503.[Accession |P40200-2] Molecular Weight The protein has a predicted MW of 54.7 kDa. Due to glycosylation, the protein migrates to 115-130 kDa based on Tris-Bis PAGE result. 内毒素Endotoxin Less than 1EU per ug by the LAL method. Purity > 95% as determined by Tris-Bis PAGEandHPLC Activity ELISA Data: Immobilized Human CD96, His Tag at 5g/ml (100l/well) on the plate. Dose response curve for Anti-CD96 Antibody, hFc Tag with the EC50 of 0.17g/ml determined by ELISA SPR Data: Human CD155, hFc Tag captured on CM5 Chip via Protein A can bind Human CD96, His Tag with an affinity constant of 0.958 M as determined in SPR assay Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.