Recombinant Human CD40/TNFRSF5 Protein,hFc Tag

Description :
Source Recombinant Human CD40/TNFRSF5 Protein is expressed from HEK293 with hFc tag at the C-terminal. It contains Glu21-Arg193.[Accession |P25942-1] Molecular Weight The protein has a predicted MW of 45.9 kDa. Due to glycosylation, the protein migrates to 55-65 kDa based on Tris-Bis PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by Tris-Bis PAGE > 95% as determined by HPLC Activity ELISA Data: Immobilized Human CD40 Ligand Trimer, His Tag at 1g/ml (100l/Well) on the plate. Dose response curve for Human CD40, hFc Tag with the EC50 of 1.29g/ml determined by ELISA. Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.