Recombinant Human CD8 alpha/CD8A Protein,His-Avi Tag

Description :
Source Recombinant Biotinylated Human CD8 alpha/CD8A Protein is expressed from HEK293 with His tag and Avi tag at the C-terminal. It contains Ser22-Asp182.[Accession |P01732-1] Molecular Weight The protein has a predicted MW of 20.5 kDa. Due to glycosylation, the protein migrates to 30-38 kDa based on Tris-Bis PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by Tris-Bis PAGE Activity ELISA Data: Immobilized Anti-CD8 Antibody, hFc Tag at 0.5g/ml (100l/well) on the plate. Dose response curve for Biotinylated Human CD8 alpha, His Tag with the EC50 of 6.0ng/ml determined by ELISA. Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.