Biotinylated Human Siglec-8 Protein, His-Avi Tag

Description :
FAQ COA 已发表文献 Siglec-8, also known as SAF, is an approximately 75kDa transmembrane glycoprotein in the Siglec family of sialic acid-binding immune regulatory molecules. Mature human Siglec-8 consists of a 347 amino acid (aa) extracellular domain (ECD) with three Ig-like domains.Putative adhesion molecule that mediates sialic-acid dependent binding to red blood cells. Preferentially binds to alpha-2,3-linked sialic acid. Also binds to alpha-2,6-linked sialic acid. 产品性质 Synonyms CDw329; MGC59785; SAF2; SAF2SAF-2; Siglec-8; SIGLEC8L; SIGLEC8 Uniprot No. Q9NYZ4 Source Recombinant Biotinylated Human Siglec-8 Protein is expressed from HEK293 Cells with His tag and Avi tag at the C-terminal. It contains Met17-Ala363. Molecular Weight The protein has a predicted MW of 40.7 kDa. Due to glycosylation, the protein migrates to 50-60 kDa based on Tris-Bis PAGE result. Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder. Purity > 95% as determined by SDS-PAGE Endotoxin 1.0EU per 1g of the protein by the LAL method. Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100g/mLis recommended. Dissolve the lyophilized protein in distilled water.