Recombinant Human LRP-5 Protein, hFc Tag

Description :
FAQ COA 已发表文献 The LDL receptor-related proteins LRP-5 and LRP-6 constitute a distinct subgroup of the LDL receptor family. Both are widely expressed type I transmembrane proteins that function as Wnt co-receptors with Frizzled proteins in the beta-cateninsignaling pathway. Both also play critical roles in gastrulation and skeletal patterning during development.LRP-5/6 associates with Wnt/Frizzled complexes, triggering phosphorylation of its intracellular PPPS motifs, binding of Axin, and propagation of beta -catenin signals. 产品性质 Synonyms LRP-5; LRP-7; LR3; BMND1; BMND1OPTA1; EVR1; EVR4; HBM; LR3VBCH2; LRP5; LRP7; OPPG; OPS; OPTA1; VBCH2 Uniprot No. O75197-1 Source Recombinant Human LRP-5Protein is expressed from Expi293 with hFc tag at the C-terminal. It contains Glu644-Gln1263. Molecular Weight The protein has a predicted MW of 96.51 kDa. Due to glycosylation, the protein migrates to 100-115 kDa based on Tris-Bis PAGE result. Purity > 95% as determined by SDS-PAGEand HPLC. Endotoxin 1.0EU per 1g of the protein by the LAL method. Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.