Biotinylated Recombinant Human Tim-3/HAVCR2 ,His -Avi Tag

Description :
FAQ COA 已发表文献 TIM-3 is a member of the TIM family of immune regulating molecules. TIMs are type I transmembrane glycoproteins with one Ig-like V-type domain and a Ser/Thr-rich mucin stalk region. Within the ECD, human TIM-3 shares 58% aa sequence identity with mouse and rat TIM-3. TIM-3 is up_x005f_x001e_regulated on several populations of activated myeloid cells (macrophage, monocyte, dendritic cell, microglia, mast cell) and T cells (Th1, CD8+, NK, Treg). Its binding to Galectin-9 induces a range of immunosuppressive functions which enhance immune tolerance and inhibit anti-tumor immunity. 产品性质 Synonyms CD366, TIM3, TIM-3, TIMD-3, TIM 3, FLJ14428, HAVCR2, KIM3 Uniprot No. Q8TDQ0-1 Source Recombinant Biotinylated Human Tim-3 Protein is expressed from HEK293Cellswith His tag and Avi tag at the C-terminal. It contains Ser22-Arg200. Molecular Weight The protein has a predicted MW of 22.8 kDa. Due to glycosylation, the protein migrates to 45-50 kDa based on Tris-Bis PAGE result. Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder. Purity > 95% as determined by SDS-PAGEand HPLC Endotoxin 1.0EU per 1g of the protein by the LAL method. Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 g/mL`is recommended (usually we use 1mg/mLsolution for lyophilization). Dissolve the lyophilized protein in distilled water.