Recombinant Human IL-25/IL-17E Protein,His Tag

Description :
Source Recombinant Human IL-25/IL-17E Protein is expressed from HEK293 with His tag at the C-terminal. It contains Tyr33-Gly177.[Uniprot|Q9H293-1] Molecular Weight The protein has a predicted MW of 17.6 kDa. Due to glycosylation, the protein migrates to 25-30 kDa based on Tris-Bis PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Activity SPR Data: Human IL-17RB, His Tag immobilized on CM5 Chip can bind Human IL-25, His Tag with an affinity constant of 0.81nM as determined in SPR assay Purity > 95% as determined by Tris-Bis PAGEand HPLC Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.