Biotinylated Human Nectin-4 Protein, His-Avi Tag

Description :
FAQ COA 已发表文献 Nectin-4 (poliovirus receptor-like 4) is a 66kDa typeI transmembrane glycoprotein belonging to the Nectin family of Ig superfamily proteins. Human Nectin-4 mRNA is normally expressed in the placenta, especially in endothelial cells, while in the mouse it is found in the embryo, lung, testis and brain. In many human ductal breast or non-small cell lung carcinomas, Nectin_x005f_x001e_4 is upregulated and a soluble 43kDa form is found in the plasma. The extracellular domain of human Nectin-4 shares 91%, 92%, 93%, 91% and 90%amino acid sequence homology with the corresponding regions of mouse, rat, canine, porcine and bovine Nectin-4, respectively. In forming adherens junctions,transinteractions of Nectin-4 initiate cell-cell interactions and recruit intracellular cadherins through afadin and other junctional proteins. 产品性质 Synonyms EDSS1; LNIR; Nectin4; PRR4; NECTIN4; PVRL4 Uniprot No. Q96NY8 Source Recombinant Biotinylated Human Nectin-4 Protein is expressed from HEK293 Cells with His tag and Avi tag at the C-terminal. It contains Gly32-Val351. Molecular Weight The protein has a predicted MW of 37.2 kDa. Due to glycosylation, the protein migrates to 46-48 kDa based on Tris-Bis PAGE result. Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder. Purity > 95% as determined by SDS-PAGE and HPLC Endotoxin 1.0EU per 1g of the protein by the LAL method. Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100g/mLis recommended. Dissolve the lyophilized protein in distilled water.