Biotinylated Human CD4/LEU3 Protein, His-Avi Tag

Description :
FAQ COA 已发表文献 CD4, also known as LEU3 is an approximately 55kDa typeI transmembrane glycoprotein that is expressed predominantly on thymocytes and a subset of mature Tlymphocytes. Within the ECD, human CD4 shares approximately 52%aa sequence identity with mouse and rat CD4. CD4 is expressed along with CD8 on double positive Tcells during their development in the thymus. CD4 binds directly to MHC classII molecules on antigen presenting cells. This interaction contributes to the formation of the immunological synapse which is focused around the TCR-MHC class II-antigenic peptide interaction. CD4 also functions as a chemotactic receptor for IL-16 and, in human, as a co-receptor for the gp120 surface glycoprotein of HIV-1. 产品性质 Synonyms CD4 antigen (p55); CD4 molecule; CD4 receptor; CD4; CD4mut; EU3 Uniprot No. P01730 Source Recombinant Biotinylated Human CD4/LEU3 Protein is expressed from HEK293 Cells with His tag and Avi tag at the C-terminal. It contains Lys26-Trp390. Molecular Weight The protein has a predicted MW of 43.6 kDa. Due to glycosylation, the protein migrates to 50-60 kDa based on Tris-Bis PAGE result. Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder. Purity > 95% as determined by SDS-PAGE and HPLC Endotoxin 1.0EU per 1g of the protein by the LAL method. Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100g/mLis recommended. Dissolve the lyophilized protein in distilled water.