Biotinylated Recombinant Human EGFR/HER1 Protein, His-Avi Tag

Description :
FAQ COA 已发表文献 The epidermal growth factor receptor is a transmembrane protein that is a receptor for members of the epidermal growth factor family of extracellular protein ligands. The EGFR subfamily of receptor tyrosine kinases comprises four members: EGFR, HER2, HER3and HER4.Within the ECD, human EGFR shares 88% aa sequence identity with mouse and rat EGFR. EGFR binds a subset of the EGF family ligands, including EGF, amphiregulin, TGF-alpha, betacellulin, epiregulin, HB-EGF, and epigen. EGFR signaling regulates multiple biological functions including cell proliferation, differentiation, motility, and apoptosis. 产品性质 Synonyms ErbB; EC 2.7.10; EC 2.7.10.1; EGFR; mENA; LEGFR; ERBB; ERBB1; HER1; PIG61; NISBD2 Uniprot No. P00533-1 Source Recombinant Biotinylated Human EGFR/HER1 Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal. It contains Leu25-Ser645. Molecular Weight The protein has a predicted MW of 71.5 kDa. Due to glycosylation, the protein migrates to 90-120 kDa based on Tris-Bis PAGE result. Purity > 95% as determined by SDS-PAGEand HPLC Activity SPR data: Biotinylated Human EGFR, His-Avi Tag captured on CM5 Chip via Anti-his antibody can bind Human EGF, No Tag with an affinity constant of 0.64 nM as determined in SPR assay. Endotoxin 1.0EU per 1g of the protein by the LAL method. Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 g/mL`is recommended (usually we use 1mg/mLsolution for lyophilization). Dissolve the lyophilized protein in distilled water.