Recombinant Human B7-H7/HHLA2 Protein,hFc Tag

Description :
Source Recombinant Human B7-H7/HHLA2 Protein is expressed from HEK293 with hFc tag at the C-terminal. It contains Ile23-Asn344.Uniprot|Q9UM44-1] Molecular Weight The protein has a predicted MW of 63.7 kDa. Due to glycosylation, the protein migrates to 78-100 kDa based on Tris-Bis PAGE result. 内毒素Endotoxin Less than 1EU per ug by the LAL method. Purity > 95% as determined by Tris-Bis PAGEand HPLC Activity ELISA Data: Immobilized Human CD28H, His Tag at 2g/ml (100l/well) on the plate. Dose response curve for Human B7-H7, hFc Tag with the EC50 of 52.7ng/ml determined by ELISA SPR Data: Human B7-H7, hFc Tag captured on CM5 Chip via Protein A can bind Human KIR3DL3, His Tag with an affinity constant of 334 nM as determined in SPR assay Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.