Recombinant Human Fas/TNFRSF6/CD95 (hFc Tag)

Description :
FAQ COA 已发表文献 Fas, also known as APO1 or CD95, belongs to the death receptor subfamily of the TNF receptor superfamily and is designated TNFRSF6. All appear to block the extrinsic apoptosis pathway induced by the Fas ligand (FasL, TNFSF6), a type II transmembrane protein of the TNF family that can be expressed on activated Tlymphocytes, NK cells and cells in immune privileged sites, or shed in soluble form. Engagement of FAS induces oligomerization of preformed Fas trimers. The activated receptor recruits the adaptor molecule FADD to form the DeathInducing Signaling Complex (DISC). Upon activation, caspases in the DISC initiate the apoptotic signaling cascade. Fas is prominent in epithelial cells, hepatocytes, activated mature lymphocytes, virus-transformed lymphocytes and tumor cells. It is an essential mediator in the activationinduced death of T lymphocytes that terminates the immune reaction. In immuneprivileged tissues, infiltrating Fasbearing lymphocytes and inflammatory cells are killed by FasL engagement. Both humans and mice with genetic defects in Fas accumulate abnormal lymphocytes and develop systemic autoimmunity. The Fas pathway also appears to crosscommunicate with the BIM (mitochondrial/intrinsic) apoptosis pathway. 产品性质 别名 FASLG receptor; CD95; APT1; FAS1; TNFRSF6; FAS; ALPS1A; APO1; FASTM; FasR Uniprot No. P25445.1 Recombinant Human Fas/TNFRSF6/CD95Protein is expressed from HEK293Cells with hFc tag at the C-terminal. It contains Gln26-Asn173. 分子量 Approximately 43.4 kDa. Due to glycosylation, the protein migrates to 55-70 kDa based on Tris-Bis PAGE result. > 95% as determined by SDS-PAGEand HPLC. ELISA DataImmobilized Human Fas Ligand, His Tag at 2g/ml (100l/Well) on the plate. Dose response curve for Human Fas, hFc Tag with the EC50 of 0.12g/ml determined by ELISA. 内毒素 1.0EU per 1g of the protein by the LAL method. Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. 复溶 Centrifuge tubes before opening. Reconstituting to a concentration more than 100 g/mL is recommended (usually we use 1mg/mLsolution for lyophilization). Dissolve the lyophilized protein in distilled water.