Recombinant Human CD89 (His Tag)

Description :
FAQ COA 已发表文献 FCAR, also called Fc alpha RI or CD89, is a variably glycosylated 50-100 kDa myeloid-specific type I transmembrane (TM) Fc receptor for IgA that is a member of the multichain immune recognition receptor (MIRR) family. Shedding and circulation of polymeric IgA/FCAR immune complexes has been reported. Circulating neutrophils, eosinophils, and monocytes express FCAR. Tissue expression of FCAR is mainly from neutrophils; FCAR is downregulated as monocytes differentiate to tissue macrophages. On neutrophils, a significant amount of FCAR lacks FcR gamma, but can still be endocytosed to early endosomes and recycled to the cell surface. Binding of serum IgA to FCAR is transient and anti-inflammatory, inhibiting IgG or IgE-induced degranulation. Sustained aggregation of FCAR results in inflammatory responses. FcR gamma signaling is required for these and for transport to late endosomes. FCAR structure resembles the KIR/ILT/LIR/MIR family more than other IgA receptors, including pIgR, Fc alpha /R, asialoglycoprotein receptor (ASGR1) and transferrin receptor (TfR). 产品性质 别名 IgA Fc receptor; CD89; FCAR; CTB-61M7.2; FcalphaRI; Fc fragment of IgA Uniprot No. Q9UIB8.1 Recombinant Human CD89Protein is expressed from HEK293Cellswith His tag at the C-terminal. It contains Gln22-Asn227. 分子量 Approximately 24.6 kDa. Due to glycosylation, the protein migrates to 45-65 kDa based on Tris-Bis PAGE result. > 95% as determined by SDS-PAGEand HPLC. 内毒素 1.0EU per 1g of the protein by the LAL method. Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. 复溶 Centrifuge tubes before opening. Reconstituting to a concentration more than 100 g/mL is recommended (usually we use 1mg/mLsolution for lyophilization). Dissolve the lyophilized protein in distilled water.