Recombinant Human CD28H/IGPR-1 (His Tag)

Description :
FAQ COA 已发表文献 CD28 homolog (CD28H), also called TMIG2 and IGPR-1, is a 55 kDa glycosylated transmembrane protein that shares approximately 10% amino acid (aa) sequence identity with CD28, CTLA-4, ICOS, and PD-1. CD28H is composed of a single extracellular immunoglobulin variable-like domain (IgV) (aa 23-109), a transmembrane domain (aa 151-171), and a long cytoplasmic domain (aa172-282). CD28H is constitutively expressed on naive T and NK cells. Similar to the interaction of B7 with CD28, the interaction of CD28H with B7-H7 activates the Akt-dependent signaling cascade and promotes the proliferation and activation of newly generated peripheral effector and memory T cells. CD28H is additionally expressed in the skin and epithelium lining the lung, airway, mammary gland, and gastrointestinal tract. It regulates cellular morphology, focal adhesion contact formation, and cell migration. CD28H also participates in angiogenesis in vitro. CD28H interacts with multiple cytoskeletal proteins including Actin, Paxillin, SPIN90, CACNB2, and BPAG1. Interactions between the cytoplasmic proline-rich domain of CD28H and SPIN90 modulate the activity of CD28H in both angiogenesis and cell adhesion. 产品性质 别名 TMIGD2; IGPR1; IGPR-1; CD28H; TPC1 Uniprot No. Q96BF3.1 Recombinant CD28H/IGPR-1Protein is expressed from HEK293Cellswith His tag at the C-terminal. It contains Leu23-Gly150. 分子量 Approximately 15.1 kDa. Due to glycosylation, the protein migrates to 42-52 kDa based on Tris-Bis PAGE result. > 95% as determined by SDS-PAGEand HPLC. ELISAData: Immobilized Human CD28H, His Tag at 2g/ml (100l/well) on the plate. Dose response curve for Human B7-H7, hFc Tag with the EC50 of 0.06g/ml determined by ELISA. 内毒素 1.0EU per 1g of the protein by the LAL method. Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. 复溶 Centrifuge tubes before opening. Reconstituting to a concentration more than 100 g/mL is recommended (usually we use 1mg/mLsolution for lyophilization). Dissolve the lyophilized protein in distilled water.