Recombinant Mouse C-Reactive/CRP (His Tag)

Description :
FAQ COA 已发表文献 C-reactive protein (CRP) is a member of the pentraxin family of plasma proteins that are part of the lectin fold superfamily of calcium-dependent, carbohydrate-binding proteins. CRP is named for its ability to bind to the C-polysaccharide of Strep. Pneumoniae. CRP is characterized by cyclic pentameric structure that contains five identical protomers/subunits, each exhibiting a lectin fold composed of two antiparallel beta -sheets with a fattened jellyroll topology. The mouse CRP precursor is 225 amino acids (aa) in length and contains a signal peptide of 19 aa with a mature polypeptide of 206 aa. There is one intrachain disulfide bond and no N-linked glycosylation site(s). Although rat CRP is glycosylated at an N-linked site, human, mouse and rabbit CRP all appear to be non-glycosylated. In mouse, the protomers are assembled non-covalently to form the pentameter; in rat, two of the five protomers are covalently linked. In mouse, IL-6 has very little effect. Mouse CRP induction is due principally to IL-1, with another pentraxin, SAP, being IL-6 inducible. CRP exhibits calcium-dependent binding to ligands. Phosphocholine (PCh), a constituent of many bacterial and fungal cell walls, is a principal ligand of CRP. CRP will also bind to the cell membrane of injured necrotic and apoptotic cells. In this context, CRP acts as an opsonin, binding to Fc gamma RI and II, and serves as an antiinflammatory agent. 产品性质 别名 C-reactive protein; CRP; PTX1; MGC88244; pentraxin 1; PTX1MGC149895 Uniprot No. P14847 Recombinant Mouse C-Reactive Protein/CRPProtein is expressed from HEK293Cellswith His tag at the C-terminal. It contains His20-Ser225. 分子量 Approximately 24.2 kDa. Due to glycosylation, the protein migrates to 26-28 kDa based on Tris-Bis PAGE result. > 95% as determined by SDS-PAGEand HPLC. 内毒素 1.0EU per 1g of the protein by the LAL method. Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. 复溶 Centrifuge tubes before opening. Reconstituting to a concentration more than 100 g/mL is recommended (usually we use 1mg/mLsolution for lyophilization). Dissolve the lyophilized protein in distilled water.