Recombinant Human LILRB4/CD85k/ILT3 Protein,His-Avi Tag

Description :
Source Recombinant Human LILRB4/CD85k/ILT3 Protein is expressed from HEK293 with His tag and Avi tag at the C-terminal. It contains Gln22-His257.[uniprot |AAH26309] Molecular Weight The protein has a predicted MW of 28.6 kDa. Due to glycosylation, the protein migrates to 38-42 kDa based on Tris-Bis PAGE result. 内毒素Endotoxin Less than 1EU per g by the LAL method. Purity > 95% as determined by Tris-Bis PAGEandHPLC Activity ELISA Data: Immobilized Human LILRB4, His Tag at 0.5g/ml (100l/Well) on the plate. Dose response curve for Anti-LILRB4 Antibody, hFc Tag with the EC50 of 7.8ng/ml determined by ELISA SPR Data: Human APOE4, hFc Tag captured on CM5 Chip via Protein A can bind Human LILRB4, His Tag with an affinity constant of 11.87 nM as determined in SPR assay Formulation Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 g/ml is recommended. Dissolve the lyophilized protein in distilled water.