Recombinant Human DDR1 (His Tag)

Description :
FAQ COA 已发表文献 DDR1, also known as CAK, CD167a, RTK6, and TrkE, is a 120-140 kDa type I transmembrane glycoprotein that belongs to the discoidin-like domain containing subfamily of receptor tyrosine kinases. DDR1 is expressed on epithelial tissues, activated monocytes and neutrophils, and in several cancers.The discoidin-like domain mediates binding to collagens I-V. DDR1 selectively recognizes the triple helical structure of collagen. It is expressed on the cell surface as a dimer which can include different isoforms. DDR1 oligomerization enhances collagen binding and also modulates collagen fibrillogenesis. The transmembrane segment contains a leucine zipper and GxxxG motif, but neither is exclusively required for dimerization. Collagen binding induces prolonged autophosphorylation, including the NPxY motif. Collagen binding also results in the proteolytic cleavage of a tyrosine phosphorylated 60 kDa C-terminal fragment (CTF), and a 60 kDa ECD fragment. TIMP3 and TAPI-1 inhibit shedding of the ECD fragment but not the CTF. 产品性质 别名 HGK2; MCK-10; CAK; EDDR1; NEP; NTRK4; PTK3A; RTK6; TRKE; CD167a; EC 2.7.10.1 Uniprot No. Q08345.1 Recombinant Human DDR1Protein is expressed from HEK293Cellswith His tag at the C-terminal. It contains Asp21-Ala417. 分子量 Approximately 45.1 kDa. Due to glycosylation, the protein migrates to 63-67 kDa based on Tris-Bis PAGE result. > 95% as determined by SDS-PAGEand HPLC. ELISA DataImmobilized Human DDR1, His Tag at 0.5g/ml (100l/well) on the plate. Dose response curve for Anti-DDR1 Antibody, hFc Tag with the EC50 of 12.8ng/ml determined by ELISA. SPR DataHuman DDR1, His Tag captured on CM5 Chip via Anti-His Antibody can bind Native Human Collagen I protein with an affinity constant of 0.806 nM as determined in SPR assay (Biacore T200). 内毒素 1.0EU per 1g of the protein by the LAL method. Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. 复溶 Centrifuge tubes before opening. Reconstituting to a concentration more than 100 g/mL is recommended (usually we use 1mg/mLsolution for lyophilization). Dissolve the lyophilized protein in distilled water.