Recombinant Human CRTAM (His Tag)

Description :
FAQ COA 已发表文献 CRTAM (Class I-restricted T cell-associated molecule) is a nectin family member of the immunoglobulin superfamily that is expressed by activated CD8+ and NK T cells. NK activation receptor engagement, but not cytokines, also induce NK CRTAM expression. CRTAM is found in spleen, thymus, small intestine, peripheral blood, and surprisingly, in brain where it is highly expressed by Purkinje cells of the cerebellum. The high affinity of CRTAM/IGSF4 adhesion allows CRTAM to disrupt IGSF4 homotypic interactions. IGSF4 and T cell receptor co-engagement of CRTAM-expressing CD8+ cells induces increased IFN-gamma or IL-22 production. A role in cancer surveillance through NK cell-mediated rejection of IGSF4-expressing tumors has been proposed. IGSF4 is expressed broadly, including on epithelia, neurons, a subset of tonsillar B cells, and a rare splenic T zone-restricted. 产品性质 别名 CD355 antigen; CD355; CRTAM Uniprot No. O95727.1 Recombinant Human CRTAMProtein is expressed from HEK293Cellswith His tag at the C-terminal. It contains Ser18-Gly287. 分子量 Approximately 31.1 kDa. Due to glycosylation, the protein migrates to 55-70 kDa based on Tris-Bis PAGE result. > 95% as determined by SDS-PAGE. ELISA Data: Immobilized Human CRTAM, His Tag at 0.5g/ml (100l/Well) on the plate. Dose response curve for Anti-CRTAM Antibody, hFc Tag with the EC50 of 57.3ng/ml determined by ELISA. 内毒素 1.0EU per 1g of the protein by the LAL method. Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. 复溶 Centrifuge tubes before opening. Reconstituting to a concentration more than 100 g/mL is recommended (usually we use 1mg/mLsolution for lyophilization). Dissolve the lyophilized protein in distilled water.