Recombinant Human CA9/Carbonic Anhydrase IX (His-Avi Tag)

Description :
FAQ COA 已发表文献 Carbonic Anhydrase (CA) catalyzes the reversible reaction of CO2+ H2O = HCO3-+ H+, which is fundamental to many processes such as respiration, renal tubular acidification and bone resorption. Topics in the CA meeting ranged from use of CAs as markers for tumor and hypoxia in clinic, as nutritional supplement in milk, and as a tool for CO2removal and mosquito control in industry. CA9, also known as membrane antigen MN and renal cell carcinoma (RCC)-associated antigen G250, is a transmembrane enzyme expressed primarily in carcinoma cells. It is one of the best markers for hypoxia and for RCC. rhCA9 corresponds to the extracellular portion of human CA9. 产品性质 别名 CA9; CAIX; CA-IX; EC 4.2.1.1; G250; MN; P54/58N; PMW1; RCC Uniprot No. Q16790 Recombinant Human CA9/Carbonic Anhydrase IXProtein is expressed from HEK293Cellswith His tag and Avi tag at the C-terminal. It contains Gln38-Asp414. 分子量 Approximately 43.7 kDa. Due to glycosylation, the protein migrates to 50-60 kDa based on Tris-Bis PAGE result. > 95% as determined by SDS-PAGEand HPLC. ELISAData:Immobilized Human CA9, His Tag at 0.9g/ml (100l/Well) on the plate. Dose response curve for Anti-CA9 Antibody, hFc Tag with the EC50 of 18.8ng/ml determined by ELISA. 内毒素 1.0EU per 1g of the protein by the LAL method. Lyophilized from 0.22m filtered solution in 50mM Tris, 150mM NaCl (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. 复溶 Centrifuge tubes before opening. Reconstituting to a concentration more than 100 g/mL is recommended (usually we use 1mg/mLsolution for lyophilization). Dissolve the lyophilized protein in distilled water.