Recombinant Human Endoglin/CD105 (His-Avi Tag)

Description :
FAQ COA 已发表文献 Endoglin (CD105) is a 90 kDa type I transmembrane glycoprotein of the zona pellucida (ZP) family of proteins. Endoglin is highly expressed on proliferating vascular endothelial cells, chondrocytes, and syncytiotrophoblasts of term placenta, with lower amounts on hematopoietic, mesenchymal and neural crest stem cells, activated monocytes, and lymphoid and myeloid leukemic cells. Endoglin homodimers interact with TGF beta 1 and TGF beta 3 (but not TGF beta 2), but only after binding T beta RII. Similarly, they interact with activinA and BMP7 via activin type IIA or B receptors, and with BMP2 via BMPR1A/ALK3 or BMPR1B/ALK6. BMP9, however, is reported to bind endoglin directly. Endoglin modifies ligandinduced signaling in multiple ways. For example, expression of endoglin can inhibit TGF beta 1 signals but enhance BMP7 signals in the same myoblast cell line. In endothelial cells, endoglin inhibits T beta RI/ALK5, but enhances ALK1mediated activation. 产品性质 别名 CD105; Endoglin; ENG; ORW; ORW1; END; HHT1 Uniprot No. P17813.1 Recombinant Human Endoglin/CD105Protein is expressed from HEK293Cells with His tag and Avi tag at the C-terminal. It contains Glu26-Gly586. 分子量 Approximately 63.7 kDa. Due to glycosylation, the protein migrates to 70-82 kDa based on Tris-Bis PAGE result. > 95% as determined by SDS-PAGEand HPLC. ELISA DataImmobilized Human Endoglin, His Tag at 5g/ml (100l/well) on the plate. Dose response curve for Anti-Endoglin Antibody, hFc Tag with the EC50 of 13.4ng/ml determined by ELISA. 内毒素 1.0EU per 1g of the protein by the LAL method. Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. 复溶 Centrifuge tubes before opening. Reconstituting to a concentration more than 100 g/mL is recommended (usually we use 1mg/mLsolution for lyophilization). Dissolve the lyophilized protein in distilled water.