Recombinant Human DPPIV/CD26 (hFc Tag)

Description :
FAQ COA 已发表文献 Dipeptidyl peptidase 4 (DPPIV, also known as CD26) is an approximately 110 kDa serine exopeptidase that releases Xaa-Pro or Xaa-Ala dipeptides from the N-terminus of oligo- and polypeptides. DPPIV is expressed as a noncovalent homodimer on the surface of epithelial cells, endothelial cells, and activated lymphocytes, and it can be released by MMP mediated shedding. It regulates immune and endocrine function through the cleavage of multiple chemokines, growth factors, and peptide hormones. It cleaves a range of peptide hormones including Glucagon, Glucagon-like Peptides 1 and 2, GIP, GHRH, Procalcitonin, Neuropeptide Y, and Substance P. It is released from adipocytes and induces insulin resistance in adipocytes and skeletal muscle. DPPIV also cleaves many chemokines, resulting in altered chemotactic activity or impacting chemokine blockade of HIV-1 cellular infectivity depending on the chemokine target. It cleaves human GM-CSF and IL-3 and reduces their ability to promote myeloid cell development. DPPIV inhibitors are approved for therapeutic treatment in type 2 diabetes and are being explored for treatment of several additional conditions including autoimmune diseases. 产品性质 别名 ADCP2; CD26; DPPIV; DPP4; ADABP; TP103; EC 3.4.14.5 Uniprot No. P27487 Recombinant Human DPPIV/CD26Protein is expressed from HEK293Cellswith hFc tag at the N-terminal. It contains Asn29-Pro766. 分子量 Approximately 111.2 kDa. Due to glycosylation, the protein migrates to 115-130 kDa based on Tris-Bis PAGE result. > 95% as determined by SDS-PAGEand HPLC. ELISA DataImmobilized Human DPPIV, hFc Tag at 1g/ml (100l/well) on the plate. Dose response curve for Biotinylated Anti-DPPIV Antibody, hFc Tag with the EC50 of 29.3ng/ml determined by ELISA. 内毒素 1.0EU per 1g of the protein by the LAL method. Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. 复溶 Centrifuge tubes before opening. Reconstituting to a concentration more than 100 g/mL is recommended (usually we use 1mg/mLsolution for lyophilization). Dissolve the lyophilized protein in distilled water.