Recombinant Human CD94 (His-Avi Tag)

Description :
FAQ COA 已发表文献 CD94 is an approximately 25 kDa type 2 transmembrane protein that plays an important role in regulating natural killer (NK) cell activation. Human CD94 consists of a 10 amino acid (aa) cytoplasmic domain, a 21 aa transmembrane segment, and a 148 aa extracellular domain (ECD) with a stem region and one C-type lectin domain. Alternative splicing generates additional isoforms that lack either the stem region, the terminal half of the ECD, or the cytoplasmic and transmembrane regions. CD94 is expressed at varying cell surface density on NK cells during their differentiation and on a subset of CD8+ T cells. It associates intodisulfide-linked heterodimers with NKG2A/B, C, or E, and these complexes function as receptors for the nonclassical MHC class I molecule, HLA-E. Ligation of CD94-NKG2A or CD94-NKG2C on NK cells triggers inhibitory or activating signals, respectively. 产品性质 别名 CD94; KLRD1; KP43; NK cell receptor Uniprot No. Q13241.1 Recombinant Human CD94Protein is expressed from HEK293Cellswith His tag and Avi tag at the N-terminal. It contains Ser34-Ile179. 分子量 Approximately 19.8 kDa. Due to glycosylation, the protein migrates to 38-42 kDa based on Tris-Bis PAGE result. > 95% as determined by SDS-PAGEand HPLC. 内毒素 1.0EU per 1g of the protein by the LAL method. Lyophilized from 0.22m filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. 复溶 Centrifuge tubes before opening. Reconstituting to a concentration more than 100 g/mL is recommended (usually we use 1mg/mLsolution for lyophilization). Dissolve the lyophilized protein in distilled water.